Raw milk is a potential source of Staphylococcus aureus in milk and milk products, especially in the case of defective pasteurization. The main reservoir of Staphylococcus aureus seems to be the infected quarter. The present study was carried out to detect the Staphylococcus aureus from milk samples collected from the suspected milch animals for sub clinical mastitis in the teaching veterinary clinical complex, Rajendranagar. The collected milk samples were poured into Brain Heart Infusion agar and incubated at 37oC for 24 hrs. DNA templates were prepared from inoculums by boiling and snap chilling method. PCR assay was standardized for primers from thermonuclease (nuc) gene to detect Staphylococcus aureus in milk samples by using gradient PCR which gave amplification product with a molecular weight of 270 bp. Out of 80 milk samples screened for presence of Staphylococcus aureus by PCR assay, 60 % samples gave positive result for Staphylococcus aureus which indicates that the major etiological agent for subclinical mastitis is Staphylococcus aureus and causes public health problems due to consumption of defective pasteurization.
Loading....